FastQCFastQC Report
Mon 17 Dec 2018
SRR4053803.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4053803.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27432709
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGAAACAGCTTCCTGGTCAGGCCGTACAGCGTCTGATGCAGCAGGGTT1228680.44788868645819846No Hit
GAATACGGAAGCGAAACTGCCCTGCAAACTGGTGTTGAAACCGCTGGGCAC1088760.3968838804800503No Hit
GTTTCAGACCGTGCAAATCTTCAAAGGTGGTGAACGCGTGGAAGCCACCTT931850.3396857379269397No Hit
GTATAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACC851590.3104286929883593No Hit
GTATCGATATGGACTTTATGAACCTGAACCAGACTGCACATGGCGGTCGCG820430.2990699897702411No Hit
TATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCA799010.2912617926286464No Hit
GTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTT675990.24641751567444542No Hit
GGACTATACCTATCACTTCGAGAAAGGTAATGACCTGGTGCTCGGCTCCCA592410.21595023663175225No Hit
GTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTG526930.1920809206265411No Hit
CTGTACGGCCTGACCAGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGT509830.18584748593367137No Hit
CTGCTACACCATGACGCCTGCCCCACAAATCCACGGCAAAAAACGACAGAA445860.16252860772882474No Hit
GCACATGGCGGTCGCGAGTTCGGCTTCATTGGCGCGCGTATGCGTCAGCAA435630.15879948276344127No Hit
GTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGA431900.1574397920380375No Hit
GTACATGGGGGGAATTCGGCCCGGAAACCCTGCGTCAGGTCACCAACATGC428320.1561347805643256No Hit
CCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGC425610.15514690875042636No Hit
GTATTGAGCTGGGGATGAAGCGTTTCCTGGAACAAGGTGGCTTCCACGCGT414570.15112251582590694No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT409670.14933632693730686No Hit
TTCCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGTGTGTCGTTATCAATC408610.14894992689201786No Hit
GCCGTGGGACAGTATCGATATGGACTTTATGAACCTGAACCAGACTGCACA405490.14781259845682757No Hit
TATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTATAGT404650.14750639464735327No Hit
GGTCTGGTGGTGTGGCTGCACACCTTCTCCCCGGCCAAAATGTGGATCAAC402920.14687576061117405No Hit
GTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTT400390.14595350389930503No Hit
TTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTC396100.1443896773009184No Hit
CCTTTATGGAGGACTATACCTATCACTTCGAGAAAGGTAATGACCTGGTGC381140.1389363332655189No Hit
GCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGC380290.13862648417259849No Hit
TCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAA364360.13281954764292508No Hit
TTTCAATGTCGTGCATCTCGGCGAATTGGCGCATATCGTTGAGGTTCAGTG363060.13234566079492915No Hit
GATCAAGTTCGGTTTCTCCGTCAATACCTGGGCGGTTGGCGATCTGGTGCA353930.12901751700861916No Hit
GAGTACATGGGGGGAATTCGGCCCGGAAACCCTGCGTCAGGTCACCAACAT349180.1272860073717109No Hit
GTGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTT335950.12246329737249063No Hit
GTCAATACCTGGGCGGTTGGCGATCTGGTGCAGGTGGTGAACTCCATCAGC335530.12231019546775349No Hit
GTACGAAAGCTGCTACACCATGACGCCTGCCCCACAAATCCACGGCAAAAA319680.11653242120564908No Hit
ACGCAGAGTACATGGGGGGAATTCGGCCCGGAAACCCTGCGTCAGGTCACC318580.11613144002657558No Hit
ATACCTATCACTTCGAGAAAGGTAATGACCTGGTGCTCGGCTCCCATATGC317450.11571952299716372No Hit
GAACCAGACTGCACATGGCGGTCGCGAGTTCGGCTTCATTGGCGCGCGTAT311550.11356880576395136No Hit
AACTTGATCTGTGCGGCAACTTTATCGCCATCGGTGACCGCCACTTCACGC310820.1133027000723844No Hit
GGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCGCCATC306340.11166961308852144No Hit
GCCGTACAGCGTCTGATGCAGCAGGGTTACGGCTTTGCGGGCGAAGGCGAC297830.10856747687587105No Hit
ATCCTGCCCGCCTGATCTTCAATACCCAAACCGGCCCAGCGATTGTCGCCA286380.10439362733006062No Hit
GTCTCTAAACAGGATACCCGTCATCTGAAAGTCTGCCGATTTGGCGATAAC285590.10410564993781693No Hit
GATAGGTATAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACA277930.10131336281808698No Hit
TATCAACGCAGAGTACATGGGGGGAATTCGGCCCGGAAACCCTGCGTCAGG275230.10032913628763387No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)